Cortisol detection in fish scales by enzyme immunoassay: Biochemical and methodological validation
نویسندگان
چکیده
منابع مشابه
DETERMINATION OF SERUM CORTISOL LEVEL BY A DIREC T ENZYME IMMUNOASSAY USING PENICILLINASE AS LABEL
Serum cortisol level was measured by an enzyme immunoassay (EIA) using the enzyme penicillinase as a label without prior extraction and purification. Polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-CMO) conjugated to bovine serum albumin (BSA). This antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticos...
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concentration of a sample that gave an absorbance value within the linear range of the assay and was expressed relative to the absorbance/g of BSA (corresponding to the gradient of the calibration curve): Interference ϭ absorbance/g of aminoglycoside absorbance/g of BSA ϫ 100% The aminoglycosides gave negligible interference in the CBB assay (Ͻ0.2% relative to BSA) and failed to generate tur...
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A solid-phase enzyme immunoassay (EIA) was developed for the detection of poliovirus antigen. Rabbit and guinea pig antisera for the assay were raised against purified poliovirus type 3/Fin (strain 3/Fin/K) isolated from a fecal specimen from a meningitis patient during an outbreak of poliomyelitis in Finland in 1984. The EIA was highly specific for poliovirus type 3, and it was about 30 times ...
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hydrochlorothiazide (hct) is a diuretic agent which is shown to be effective in the treatment of hypertension. literature reports have demonstrated that urinary excretion data may be used to assess the bioavailability of various formulations containing this thiazide. also hct consumption by the athletes is one of the drugs which should be regulated by world anti-doping agency (wada), because of...
determination of serum cortisol level by a direc t enzyme immunoassay using penicillinase as label
serum cortisol level was measured by an enzyme immunoassay (eia) using the enzyme penicillinase as a label without prior extraction and purification. polyclonal antibodies were raised against cortisol-3-ortho-carboxymethyl-oxime (cortisol-3-0-cmo) conjugated to bovine serum albumin (bsa). this antibody showed a very low cross-reactivity with structurally related steroids (3.7% for corticosteron...
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ژورنال
عنوان ژورنال: Journal of Applied Ichthyology
سال: 2018
ISSN: 0175-8659
DOI: 10.1111/jai.13674